Multiplexed profiling facilitates robust m6A quantification at site, gene and sample resolution
Published in Nature Methods, 2021
Here we develop m6A-seq2, a multiplexed immunoprecipitation-RNA-seq coupled method that measures m6A across the epitranscriptome reducing technical variability, cost, and labor. Allowing for sample-level m6A relative quantitations, orthogonal to mass-spectrometry methods, as well as gene-level quantitations. We found that m6A-seq2 m6A gene-level measurements explains roughly 30% of the variability in the RNA half life on mouse embryonic stem cells.
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Recommended citation: Dierks, D., Garcia-Campos, M.A., Uzonyi, A., Safra, M., Edelheit, S., Rossi, A., Sideri, T., Varier, R.A., Brandis, A., Stelzer, Y. and van Werven, F., 2021. Multiplexed profiling facilitates robust m6A quantification at site, gene and sample resolution. Nature Methods, pp.1-8. https://www.nature.com/articles/s41592-021-01242-z